Ukukhishwa kwe-DNA/RNA

Isingeniso somkhiqizo:

Isebenzisa ubuchwepheshe bokuhlanza ubuhlalu obuzibuthe, ikhithi yokuhlanza i-Magpure virus DNA/RNA ingakhipha i-DNA/RNA yamagciwane ahlukahlukene njengegciwane le-African Swine Fever kanye ne-coronavirus yenoveli kumasampula ahlukahlukene njenge-serum, i-plasma kanye nesisombululo sokucwiliswa kweswab, futhi ingasetshenziswa ku-PCR engezansi komfula. /RT-PCR, ukulandelana, ukuhlaziywa kwe-polymorphism nokunye ukuhlaziya nokuhlolwa kwe-nucleic acid. Ifakwe NETRACTION ithuluzi lokuhlanza i-nucleic acid elizenzakalelayo ngokugcwele kanye nekhithi yokulayisha ngaphambilini, ingaqedela ngokushesha ukukhishwa kwenani elikhulu lamasampuli e-nucleic acid.

Izici Zomkhiqizo:

1. Kuphephile ukusebenzisa, ngaphandle kwe-reagent enobuthi
2. Kulula ukuyisebenzisa, asikho isidingo se-Proteinase K ne-Carrier RNA
3. Khipha i-DNA/RNA yegciwane ngokushesha nangempumelelo ngokuzwela okuphezulu
4. Ezokuthutha kanye nesitolo endaweni yezinga lokushisa.
5. Ifanele ukuhlanzwa kwe-viral nucleic acid ehlukahlukene
6. Ifakwe i-NUETRACTION ngokuzenzakalelayo ngokugcwele ithuluzi lokuhlanza i-nucleic acid ukuze kucutshungulwe amasampula angama-32 phakathi nemizuzu engama-30.